![]() HPV infection occurs in the epithelium through microscopic wounds making basal cells exposed to the virus. Subsequently, in silico cloning of the multi-epitope vaccine was conducted into pET-28a ( +) expression vector.Ĭervical cancer is the fourth most common cancer affecting women and is caused by human Papillomavirus (HPV) infections that are sexually transmitted. Codon adaptation was performed in order to achieve efficient vaccine expression in Escherichia coli strain K12 ( E. The structural stability of the vaccine construct was confirmed by molecular dynamics simulation. Molecular docking analysis showed that the vaccine's refined 3D model had a strong interaction with the Toll-like receptor 4. The vaccine’s B-cell epitopes were predicted. The vaccine’s 3D model was predicted, and the structural improvement after refinement was confirmed using the Ramachandran plot and ProSA-web. Furthermore, the vaccine was soluble, highly antigenic, and non-allergenic. The physicochemical parameters of the vaccine construct were acceptable. In addition, a C-terminal fragment of Mycobacterium tuberculosis heat shock protein 70 (HSP70) was used as an adjuvant for the vaccine construct. Then, the selected epitopes were sequentially linked by appropriate linkers. These epitopes were evaluated and chosen based on their antigenicity, allergenicity, toxicity, and induction of IFN-γ production (only in helper T lymphocytes). In this study, T-cell epitopes from E5 and E7 proteins of HPV16/18 were predicted. GUID: 33B2D9F8-5F70-4FB1-B3A1-50DB72EBB766 Data Availability Statementĭatasets used in the experiments are listed as follows: (1) NCBI. The Creative Commons Public Domain Dedication waiver ( ) applies to the data made available in this article, unless otherwise stated in a credit line to the data. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. ![]()
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